Premium
Conjugated Linoleic Acids Can Change Phagocytosis of Human Monocytes/Macrophages by Reduction in Cox‐2 Expression
Author(s) -
Stachowska Ewa,
BaśkiewiczMasiuk Magdalena,
Dziedziejko Violetta,
Adler Grażyna,
Bober Joanna,
Machaliński Bogusław,
Chlubek Dariusz
Publication year - 2007
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-007-3072-2
Subject(s) - phagocytosis , conjugated linoleic acid , monocyte , macrophage , prostaglandin , prostaglandin e , biochemistry , chemistry , microbiology and biotechnology , biology , linoleic acid , in vitro , immunology , fatty acid
Prostaglandin E 2 produced endogenously (by cyclooxygenases) can regulate macrophage phagocytosis. Cyclooxygenase activity reduction (mainly through inhibition of inducible Cox‐2) can induce PGE 2 synthesis depression and can activate the phagocytosis process. There are no reports in the literature explaining whether conjugated linoleic acid dienes ( trans‐ 10, cis ‐12 CLA and cis ‐9, trans‐ 11 CLA) modify the phagocytic activity of human macrophages. For the purpose of this study, monocytes were isolated from venous blood, incubated for 7 days with 30 μM CLAs, and then (in some experiments) LPS (1 μg/mL) was added to the medium. Subsequently, monocyte/macrophage phagocytosis, NF‐κB transcription factor activity, Cox‐2 and PPARγ mRNA expression (and the amounts of Cox‐2 and PPARγ proteins) and PGE 2 synthesis were determined. Both CLA isomers increased macrophage phagocytosis through inhibition of Cox‐2 expression (might by inactivation the NF‐κB pathway). The inhibition of mRNA Cox‐2 expression contributed (particularly with respect to trans‐ 10, cis‐ 12 CLA) to a decrease in protein Cox‐2 synthesis and to reduction of prostaglandin E 2 content in the cell. The inhibition of PGE 2 synthesis (by CLA treatment) enhanced the phagocytosis process in macrophages.