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Rapid Analysis of Acylglycerols in Low Molecular Weight Milk Fat Fractions
Author(s) -
Craven R. J.,
Lencki R. W.
Publication year - 2007
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-007-3044-6
Subject(s) - glyceride , lipidology , clinical chemistry , chemistry , chromatography , food science , diglyceride , biochemistry , fatty acid
A suitable analytical method was required to facilitate development of an industrial‐scale short‐path distillation (SPD) process. Short‐path distillation produces milk fat distillates (MFD) enriched in low molecular weight milk fat components—viz. free fatty acids, monoacylglycerols, diacylglycerols, cholesterol and low molecular weight triacylglycerols. In this case, solid‐phase extraction (SPE) was considered a better alternative than thin‐layer chromatography for separating polar and apolar lipid components in MFD samples due to its speed and near‐complete recoveries. Solid‐phase extraction of MFDs yielded two fractions, both of which are sufficiently pure for subsequent analysis by gas chromatography. This procedure provided rapid and complete chemical characterization (including mass balances) of low‐molecular weight milk‐fat fractions.