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Levels of Lipid Peroxidation in Human Plasma and Erythrocytes: Comparison between Fatty Acids and Cholesterol
Author(s) -
Yoshida Yasukazu,
Saito Yoshiro,
Hayakawa Mieko,
Habuchi Yoko,
Imai Yasuharu,
Sawai Yoshiyuki,
Niki Etsuo
Publication year - 2007
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-007-3037-5
Subject(s) - lipid peroxidation , lipidology , chemistry , clinical chemistry , cholesterol , saponification , antioxidant , biochemistry , sodium borohydride , lipid metabolism , sodium , food science , organic chemistry , catalysis
Lipid peroxidation has gained renewed attention with increasing evidence showing its biological role in producing toxic compounds and cellular signaling mediators. The assessment of lipid peroxidation levels in vivo is difficult partly because lipids are oxidized by different oxidants by different mechanisms to give versatile types of products, which may undergo metabolism and secondary reactions. In the present study, total hydroxyoctadecadienoic acids (tHODE) and 7α‐ and 7β‐hydroxycholesterol (t7‐OHCh) from 44 healthy human subjects were assessed as biomarkers after reduction with sodium borohydride followed by saponification with potassium hydroxide comparing with the prevailing standard 8‐isoprostaglandin F 2α (t8‐iso‐PGF 2α ). The average concentrations of tHODE, total 8‐isoprostaglandin F 2α (t8‐iso‐PGF 2α ), t7α‐OHCh, and t7β‐OHCh were 203, 0.727, 87.1, and 156 nmol/l plasma and 1,917, 12.8, 1,372, and 3,854 nmol/l packed erythrocytes, respectively. The ratios of tHODE and t7‐OHCh to the parent substrates were 194 and 3,519 μmol tHODE/mol linoleates and 40.9 and 686 μmol t7‐OHCh/mol cholesterol in plasma and erythrocytes, respectively. It was found that (1) t7‐OHCh in blood was unexpectedly high, as high as or even higher than tHODE, (2) the amounts of tHODE was more than 100 fold higher than t8‐iso‐PGF 2α (3) the level of lipid oxidation products in erythrocytes was higher than that in plasma, and (4) lipid peroxidation products level tended to increase while antioxidant level decrease with age. These products may be used as potential biomarker for assessment of lipid peroxidation and oxidative stress in vivo.