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Rapid Desensitization of Lipolysis in the Visceral and Subcutaneous Adipocytes of Rats
Author(s) -
Mori Shinobu,
Nojiri Hiroshi,
Yoshizuka Naonobu,
Takema Yoshinori
Publication year - 2007
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-007-3034-8
Subject(s) - lipolysis , endocrinology , medicine , agonist , stimulation , chemistry , terbutaline , adipose tissue , desensitization (medicine) , adipocyte , receptor , clinical chemistry , biology , asthma
In adipocytes, short and long term stimulation of β adrenergic receptors (βAR) induces the desensitization to catecholamines, leading to a decrease in the intracellular accumulation of cAMP, but the roles played by this in lipolysis is not clear. In this study, we assessed the catecholamine‐induced desensitization of lipolysis and compared this in adipocytes isolated from visceral and subcutaneous fat tissues of rats. When adipocytes were pretreated with isoproterenol (ISO), the norepinephrine (NE)‐induced lipolysis was significantly reduced dose‐ and time‐dependently. A similar reduction of the lipolytic response was also found in NE‐, dobutamine‐, terbutaline‐ or BRL37344‐induced lipolysis. The ISO‐ and each βAR agonist‐induced lipolysis in the visceral fat was not only higher than in the subcutaneous fat, but also markedly reduced by ISO‐ or NE‐pretreatment. These results showed that short‐term treatment of three subtypes of βAR by each agonist induces a rapid reduction in the lipolytic response to βAR stimulation. This suggests some common mechanism for the rapid desensitization of βAR‐agonist‐induced lipolysis, in contrast with previous reports on the characteristics of βAR subtypes. In addition, the regional difference of adipose tissue not only in inducing lipolysis but also in rapid desensitization was also apparent.