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Differential biohydrogenation and isomerization of [U‐ 13 C]Oleic and [1‐ 13 C]Oleic acids by mixed ruminal microbes
Author(s) -
Mosley Erin E.,
Nudda Anna,
Corato Adolfo,
Rossi Erica,
Jenkins Tom,
McGuire Mark A.
Publication year - 2006
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-006-5125-3
Subject(s) - oleic acid , isomerization , chemistry , clinical chemistry , metabolism , stearic acid , biochemistry , food science , organic chemistry , catalysis
The additional mass associated with 13 C in metabolic tracers may interfere with their metabolism. The comparative isomerization and biohydrogenation of oleic, [1‐ 13 C]oleic, and [U‐ 13 C]oleic acids by mixed ruminal microbes was used to evaluate this effect. The percent of stearic, cis ‐14 and‐ 15, and trans ‐9 to‐16 18∶1 originating from oleic acid was decreased for [U‐ 13 C]oleic acid compared with [1‐ 13 C]oleic acid. Conversely, microbial utilization of [U‐ 13 C]oleic acid resulted in more of the 13 C label in cis ‐9 18∶1 compared with [1‐ 13 C]oleic acid (53.7 vs. 40.1%). The isomerization and biohydrogenation of oleic acid by ruminal microbes is affected by the mass of the labeled tracer.