Premium
Regiospecificity profiles of storage and membrane lipids from the gill and muscle tissue of atlantic salmon ( Salmo salar L.) grown at elevated temperature
Author(s) -
Miller Matthew R.,
Nichols Peter D,
Barnes Julia,
Davies Noel W.,
Peacock Evan J.,
Carter Chris G.
Publication year - 2006
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-006-5042-5
Subject(s) - salmo , polyunsaturated fatty acid , chemistry , muscle tissue , membrane lipids , fatty acid , food science , zoology , lipidology , biology , biochemistry , membrane , fishery , fish <actinopterygii> , anatomy
Regiospecific and traditional analysis, of both storage and membrane lipids, was performed on gill, white muscle, and red muscle samples taken from Atlantic salmon ( Salmo salar ) to gauge the effect of elevated water temperature. The fish, fed a commercial diet, were held at an elevated water temperature of 19°C. Total n‐3 PUFA, total PUFA, and n‐3/n‐6 and unsaturated/saturated fatty acid (UFA/SFA) ratios in the FA profile of the total lipid extract in the white muscle were fairly low compared with fish grown at 15°C. Adaptation of structural and storage lipids at elevated temperatures was shown by a significant ( P <0.01) reduction in PUFA especially in the percentage of EPA (6–8%). Further adaptation was indicated by the percentages of SFA, which were significantly ( P <0.05) higher in gill (56%) and white muscle (58%) polar lipid fractions and coincided with lower percentages of n‐3, n‐6, and total PUFA. The regiospecific profiles indicated a high affinity of DHA to the sn ‐2 position in both the TAG (61–68%) and polar lipid (35–60%) fractions. The combination of detailed regiospecific and lipid analyses demonstrated adaptation of cell membrane structure in Atlantic salmon grown at an elevated water temperature.