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Butters Varying in trans 18:1 and cis ‐9, trans‐ 11 Conjugated Linoleic Acid Modify Plasma Lipoproteins in the Hypercholesterolemic Rabbit
Author(s) -
Bauchart Dominique,
Roy Alexandre,
Lorenz Stephanie,
Chardigny JeanMichel,
Ferlay Anne,
Gruffat Dominique,
Sébédio JeanLouis,
Chilliard Yves,
Durand Denys
Publication year - 2007
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-006-3018-0
Subject(s) - conjugated linoleic acid , very low density lipoprotein , lipidology , chemistry , apolipoprotein b , clinical chemistry , medicine , endocrinology , cholesterol , lipoprotein , radial immunodiffusion , low density lipoprotein , linoleic acid , biochemistry , fatty acid , biology , antibody , immunology
The experiment was designed to study the effects of butters differing in conjugated linoleic acid (CLA) and trans 18:1 contents on lipoproteins associated with the risk of atherogenesis. New Zealand White male rabbits (9.6 weeks; 2.1 kg) were assigned for 6 or 12 weeks to three diets ( n = 6 per diet) made of conventional pellets with 0.2% cholesterol and with 12% fat provided from a butter poor in trans ‐10 and trans ‐11 18:1 and in CLA (standard group), or rich in trans‐ 10 18:1 ( trans ‐10 18:1 group) or rich in trans ‐11 18:1 and in cis ‐9, trans ‐11 CLA ( trans ‐11 18:1/CLA group). Blood samples were collected at the end of dietary treatments. Lipoproteins were separated by gradient‐density ultracentrifugation. Lipid classes were determined enzymatically and apolipoproteins A‐I and B by radial immunodiffusion. Mainly in the 12‐week rabbits, higher plasma triglycerides and apolipoprotein B levels shown in the standard and trans ‐10 18:1 groups compared with those in the trans ‐11 18:1/CLA group are associated with higher plasma levels of very low density lipoproteins (VLDL) and low density lipoproteins (LDL) also shown in these two groups. In the 12‐week rabbits, a shift towards denser LDL, considered as more atherogenic, was shown only in the trans ‐10 18:1 group. In these animals, the VLDL + LDL to HDL ratio was 1.7–2.3 times higher in the trans ‐10 18:1 group than in the other groups ( P = 0.076). These results suggest a rather neutral effect of trans ‐11 18:1/CLA butter towards the risk of atherogenesis, whereas trans ‐10 18:1 butter would tend to be detrimental.