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Inhibition of 12‐ and 15‐lipoxygenase activities and protection of human and tilapia low density lipoprotein oxidation by I‐Tiao‐Gung ( Glycine tomentella )
Author(s) -
Chen TsuiYao,
Shiao MingShi,
Pan Bonnie Sun
Publication year - 2005
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-005-1482-1
Subject(s) - trolox , antioxidant , chemistry , ic50 , lipoxygenase , milt , pharmacology , nordihydroguaiaretic acid , platelet rich plasma , biochemistry , cyclooxygenase , endocrinology , medicine , platelet , enzyme , dpph , in vitro , andrology , sperm
I‐Tiao‐Gung, Glycine tomentella , has been used extensively as a traditional herbal medicine to relieve physical pain, but its bioactivity has not been studied systematically. Ninety‐five percent ethanol extracts of G. tomentella (GT‐E) showed antioxidant activity in human plasma by prolonging the lag phase (+T lag ) of Cu 2+ ‐induced, LDL oxidation and were dose dependent. The+T lag of LDL combined with 3.2 μg/mL GT‐E was similar to that with 2.0 μM ( ca . 0.5 μg/mL) Trolox. A similar inhibitory effect was found toward tilapia plasma LDL. In addition, GT‐E inhibited tilapia thrombocyte (nucleated platelet), 5‐, 12‐, and 15‐lipoxygenase (LOX). The IC 50 values were 0.43, 0.72, and 0.42 μg/mL, respectively, whereas the IC 50 values for nordihydroguaiaretic acid (NDGA) on 5‐, 12‐, and 15‐LOX were 2.3, 1.6, and 1.7 μg/mL, respectively. The IC 50 value for cyclooxygenase‐2 (COX‐2) inhibition by GT‐E was 42.0 μg/mL, whereas the IC 50 value by indomethacin as a positive control was 0.61 μg/mL. The prevention of LDL oxidation and the dual inhibition of LOX and COX‐2 are indicative of the possible roles of I‐Tiao‐Gung in antiatherosclerosis and anti‐inflammation.