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Comparative effects of palm vitamin E and α‐tocopherol on healing and wound tissue antioxidant enzyme levels in diabetic rats
Author(s) -
Musalmah M.,
Nizrana M. Y.,
Fairuz A. H.,
NoorAini A. H.,
Azian A. L.,
Gapor M. T.,
Wan Ngah W. Z.
Publication year - 2005
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-005-1418-9
Subject(s) - antioxidant , malondialdehyde , superoxide dismutase , glutathione peroxidase , chemistry , vitamin e , lipid peroxidation , wound healing , vitamin c , biochemistry , pharmacology , medicine , surgery
The effect of supplementing 200 mg/kg body weight palm vitamin E (PVE) and 200 mg/kg body weight α‐tocopherol (α‐loc) on the healing of wounds in streptozotocin‐induced diabetic rats was evaluated. The antioxidant potencies of these two preparations of vitamin E were also evaluated by determining the antioxidant enzyme activities, namely, glutathione peroxidase (GPx) and superoxide dismutase (SOD), and malondialdehyde (MDA) levels in the healing of dermal wounds. Healing was evaluated by measuring wound contractions and protein contents in the healing wounds. Cellular redistribution and collagen deposition were assessed morphologically using cross‐sections of paraffin‐embedded day‐10 wounds stained according to the Van Gieson method. GPx and SOD activities as well as MDA levels were determined in homogenates of day‐10 dermal wounds. Results showed that PVE had a greater potency to enhance wound repair and induce the increase in free radical‐scavenging enzyme activities than α‐Toc. Both PVE and α‐Toc, however, were potent antioxidants and significantly reduced the lipid peroxidation levels in the wounds as measured by the reduction in MDA levels.