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Foam fractionation of exo‐lipases from a growing fungus ( Pleurotus sapidus )
Author(s) -
Linke Diana,
Zorn Holger,
Gerken Birte,
Parlar Harun,
Berger Ralf G.
Publication year - 2005
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-005-1389-x
Subject(s) - fractionation , chromatography , foam fractionation , pleurotus , chemistry , pellet , fungus , mycelium , hydrolysis , food science , biology , botany , biochemistry , mushroom , pulmonary surfactant , zoology
Active lipases were isolated from the culture supernatant of the basidiomycetous fungus Pleurotus sapidus by foam fractionation. The pH value, the gas flow, and the foaming period were systematically varied to optimize the transport of the enzymes into the foam phase. On a 70‐mL scale, a maximum recovery of hydrolytic activity of 95% was obtained at pH 7.0 and 60 mL N 2 min −1 after 50 min. The procedure, with minor modifications, was also applicable to native pellet cultures of P. sapidus . The same recovery of 95% was achieved, with purification and enrichment factors of 11.6 and 28.0, respectively.