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Phosphatidylcholine hydroperoxide levels in human plasma are lower than previously reported
Author(s) -
Adachi Junko,
Yoshioka Naoki,
Funae Rika,
Nagasaki Yasushi,
Naito Takaeki,
Ueno Yasuhiro
Publication year - 2004
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-004-1311-6
Subject(s) - lipidology , clinical chemistry , phosphatidylcholine , human plasma , chemistry , chromatography , biochemistry , phospholipid , membrane
The quantification of PC hydroperoxide (PCOOH) in human plasma was studied by HPLC with chemiluminescence detection (HPLC‐CL). We identified for the first time the monohydroperoxide of 1‐palmitoyl‐2‐linoleoyl‐PC hydroperoxide (PC 16∶0/18∶2‐OOH) in plasma by LC‐MS and HPLC‐CL. The standard compound, PC 16∶0/18∶2‐OOH (synthetic PCOOH), as well as PCOOH from egg yolk, was used. Comparison of the PCOOH concentration in each participant's plasma as determined by use of a Finepak SIL NH 2 column with 2‐propanol/methanol/water as the mobile phase (system A, the conventional method) gave a higher concentration than did an LC‐18‐DB column with methanol containing 0.01% triethylamine (system B). The mean PCOOH concentration for the 43 healthy volunteers was 55.1±30.4 pmol/mL (mean±SD) for system A and 16.3±9.9 pmol/mL for system B. Moreover, the main peak of the plasma extract appeared at a different time from that of synthetic PCOOH or egg yolk PCOOH in system A, whereas in system B plasma sample retention time practically corresponded to that of standard PCOOH. These findings confirm that the PCOOH plasma concentration is not so high as previously reported.