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Successful utilization of lyophilized lipoprotein(a) as a biological reagent
Author(s) -
Scanu Angelo M.,
Hinman Janet,
Pfaffinger Ditta,
Edelstein Celina
Publication year - 2004
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-004-1268-5
Subject(s) - lipidology , clinical chemistry , reagent , chemistry , lipoprotein , chromatography , biochemical engineering , biochemistry , cholesterol , organic chemistry , engineering
Lipoprotein(a) [Lp(a)] represents a class of lipoprotein particles having as a protein moiety apoB‐100 linked by a single disulfide bond to apolipoprotein(a) [apo(a)], a multikringle structure with a high degree of homology with plasminogen. A recognized feature of Lp(a) is its instability on storage caused by attendant protein and lipid modifications that affect the structural, functional, and immunological properties of this lipoprotein. Here we present data showing that, under appropriate conditions of cryopreservation, Lp(a) retains the properties of the freshly isolated product, and we provide examples supporting the stability of this cryopreserved product as a primary standard in immunoassay settings and in cell culture systems.