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The up‐regulation of hepatic acyl‐coA oxidase and cytochrome P 450 4A1 mRNA expression by dietary oxidized frying oil is comparable between male and female rats
Author(s) -
Chao PeiMin,
Hsu ShanChing,
Lin FuJung,
Li YiJen,
Huang Chingjang
Publication year - 2004
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-004-1224-4
Subject(s) - medicine , endocrinology , peroxisome , biology , oxidase test , peroxisome proliferator activated receptor , clinical chemistry , catalase , cytochrome p450 , lipidology , gene expression , receptor , messenger rna , sexual dimorphism , biochemistry , enzyme , gene , metabolism , oxidative stress
We previously demonstrated that oxidized frying oil (OFO) activates peroxisome proliferator‐activated receptor α (PPARα) and up‐regulates hepatic acyl‐CoA oxidase (ACO) and cytochrome P 450 4A1 (CYP4A1) genes in male rats. As female rats were shown to be less responsive to some peroxisome proliferators (PP), this study compared the expression of a few PPARα target genes in male and female rats fed diets containing OFO. Male and female rats were fed a diet containing 20 g/100 g OFO (O diet) or fresh soybean oil (F diet) for 6 wk. Both male and female rats fed the O diet showed significantly higher liver weight, hepatic ACO and catalase activities, CYP4A protein, and expression of ACO and CYP4A1 mRNA ( P <0.05) compared with their control groups. The mRNA expression of two other PPARα target genes, FA‐binding protein and HMG‐CoA synthase, were marginally increased by dietary OFO ( P =0.0669 and 0,0521, respectively). Female rats fed the O diet had significantly lower CYP4A protein than male rats fed the same diet. The remaining OFO‐induced effects were not significantly different between male and female rats fed the O diet. These results indicate that dietary OFO, unlike clofibrate or other PP, had minimal sexual dimorphic effect on the induction of hepatic PPARα target gene expression.