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β‐Oxidation of 18∶3n−3 in atlantic salmon ( Salmo salar L.) hepatocytes treated with different fatty acids
Author(s) -
Torstensen Bente E.,
Stubhaug Ingunn
Publication year - 2004
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-004-1213-7
Subject(s) - hepatocyte , salmo , catabolism , clinical chemistry , stimulation , in vitro , chemistry , lipidology , biochemistry , fatty acid , biology , metabolism , fish <actinopterygii> , endocrinology , fishery
To study whether Atlantic salmon β‐oxidation was affected by dietary FA composition, an in vitro study with primary hepatocytes was undertaken. Isolated hepatocyte cultures were stimulated with either 16∶0, 18∶1n−9, 18∶2n−6, 18∶3n−3, 20∶5n−3, or 22∶6n−3 in triplicate for 24 h. In addition, a control was included where no FA stimulation was performed, also in triplicate. After stimulation, radiolabeled [1‐ 14 C]18∶3n−3 was added and the cells were incubated for 2 h at 20°C. The cells were then harvested, and radioactivity was determined in the acid‐soluble part of the cells and medium, i.e., the end products of the β‐oxidation pathway. Specific β‐oxidation activity was significantly higher in hepatocytes stimulated with 18∶3n−3. Further, when taking into account the amount of radiolabeled [1‐ 14 C]18∶3n−3 taken up by the cells—the relative amount of β‐oxidized [1‐ 14 C]18∶3n−3 of the total FA taken up by the hepatocytes—no significant differences were found. Thus, the regulation of β‐oxidation activity in the primary Atlantic salmon hepatocytes seems to be at the level of FA uptake and transport into the cell. This in vitro study shows that the catabolism processes in salmon hepatocytes are affected by the FA available and probably already regulated at the level of FA uptake.