Premium
Two distinct pathways for the formation of hydroxy FA from linoleic acid by lactic acid bacteria
Author(s) -
Kishimoto Noriaki,
Yamamoto Izumi,
Toraishi Kazuki,
Yoshioka Satiko,
Saito Kenji,
Masuda Hideki,
Fujita Tokio
Publication year - 2003
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-003-1188-4
Subject(s) - linoleic acid , lactic acid , bacteria , lactobacillus paracasei , pediococcus , chemistry , food science , biology , fatty acid , biochemistry , genetics
Twenty‐three of 86 strains of lactic acid bacteria transformed linoleic acid into hydroxy FA. Two distinct conversion pathways were in operation. Two strains of Lactobacillus acidophilus and a strain of Pediococcus pentosaceus produced 13( S )‐hydroxy‐9‐octadecenoic acid [13( S )‐OH 18∶1] and 10,13‐dihydroxyoctadecanoic acid (10,13‐OH 18∶0) as main and minor products, respectively, whereas 13 strains, including L. casei subsp. casei, L. paracasei subsp, paracasei, L. rhamnosus, L. lactis subsp. cremoris , and Streptococcus salivarius subsp. thermophilus produced 10‐hydroxy‐12‐octadecenoic acid (10‐OH 18∶1). Seven strains of L. plantanum converted linoleic acid to 10‐hydroxyoctadecanoic acid (10‐OH 18∶0) through 10‐OH 18∶1 Linoleic acid at 2 g/L was converted by L. acidophilus LFO13951 T to 1.3 g of 13( S )‐OH 18∶1 and 0.09 g of 10,13‐OH 18∶0 in 7 d. Lactobacillus paracasei subsp. paracasei JCM 1111 produced 10‐OH 18∶1 in 91% yield, and L. plantarum JCM 8341, 10‐OH 18∶0 in 59% yield from linoleic acid (2 g/L) under optimal conditions. To our knowledge, this is the first report on the production of 13( S )‐OH 18∶1 by lactic acid bacteria other than ruminal bacteria, and of 10,13‐OH 18∶0 by any bacteria.