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Fatty acids bound to Fasciola hepatica 12 kDa fatty acid‐binding protein, a candidate vaccine, differ from fatty acids in extracts of adult flukes
Author(s) -
Carballeira Néstor M.,
Cruz Heidyleen,
Hillyer George V.
Publication year - 2003
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-003-1125-6
Subject(s) - fasciola hepatica , polyunsaturated fatty acid , fatty acid binding protein , hepatica , clinical chemistry , biochemistry , fatty acid , lipidology , chemistry , biology , gene , immunology , helminths
The FA composition of Fasciola hepatica 12 kDA purified native FA‐binding protein ( n Fh12), a candidate vaccine against fascioliasis, is described. The FA chain lengths ranged between 12 and 24 carbons. The principal FA were 16∶0 18∶1n−9, 18∶0, 20∶4n−6, and 20∶1n−9. The acids 16∶0, 18∶1n−9, and 18∶0 comprised over half the FA that were bound to the whole FA‐binding protein. Small amounts (1.0–2.8%) of isoanteiso methyl‐branched FA also were characterized. Forty‐one different FA were identified in extracts of the adult flukes, with the three most abundant FA also being 16∶0, 18∶1n−9, and 18∶0. A similar proportion of saturated vs. unsaturated FA was observed between the whole extract from F. hepatica and the n Fh12 protein. However, the n−3/n−6 ratio of PUFA was significantly different, being 1.2 in the whole extract vs. 9.6 in the n Fh12 protein complex. The n Fh12 protein binds more n−5, n−6, and n−7 PUFA and less n−3 and n−9 PUFA than the whole extract. In addition, cholesterol (56%), sitosterol (36%), and fucosterol (8%) also were bound to the n Fh12 protein complex.