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Transfer of arachidonic acid from lymphocytes to macrophages
Author(s) -
Peres C. M.,
Sampaio S. C.,
Cury Y.,
Newsholme P.,
Curi R.
Publication year - 2003
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-003-1108-7
Subject(s) - phagocytosis , incubation , arachidonic acid , linoleic acid , microbiology and biotechnology , chemistry , palmitic acid , oleic acid , cell culture , in vitro , biochemistry , biology , fatty acid , enzyme , genetics
The incorporation and oxidation of arachidonic acid (AA) by rat lymphocytes (LY), the transfer of AA from LY to rat macrophages (Mϕ) in co‐culture, and the subsequent functional impact on Mϕ phagocytosis were investigated. The rate of incorporation of [1‐ 14 C]AA by untreated‐LY and TG (thioglycolate treated)‐LY (TG‐LY) was 158±8 nmol/10 10 LY per h for both untreated‐LY and TG‐LY. The oxidation of AA was 3.4‐fold higher in TG‐LY as compared with untreated cells. LY from TG‐injected rats had a 2.5‐fold increase in the oxidation of palmitic (PA), oleic (OA), and linoleic (LA) acids. After 6 h of incubation, [ 14 C] from AA was distributed mainly into phospholipids. The rate of incorporation into total lipids was 1071 nmol/10 10 cells in untreated‐LY and 636 nmol/10 10 cells in TG‐LY. [ 14 C]AA was transferred from LY to co‐cultured Mϕ in substantial amounts (8.7 nmol for untreated and 15 nmol per 10 10 for TG cells). Exogenously added AA, PA, OA, and LA caused a significant reduction of phagocytosis by resident cells. Mϕ co‐cultured with AA‐preloaded LY showed a significant reduction of the phagocytic capacity (about 40% at 35 μM). LY preloaded with PA, LA, and OA also induced a reduction in phagocytic capacity of co‐cultured Mϕ. TG treatment abolished the AA‐induced inhibition of phagocytosis in Mϕ co‐cultured with TG‐LY. Therefore, the transfer of AA between leukocytes is a modulated process and may play an important role in controlling inflammatory and immune response.