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Cloning of an alkaline lipase gene from Penicillium cyclopium and its expression in Escherichia coli
Author(s) -
Wu Minchen,
Qian Zhikang,
Jiang Peihong,
Min Taishan,
Sun Chongrong,
Huang Weida
Publication year - 2003
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-003-1051-7
Subject(s) - open reading frame , lipase , microbiology and biotechnology , complementary dna , biology , gene , signal peptide , amino acid , biochemistry , escherichia coli , molecular cloning , peptide sequence , enzyme
The gene encoding an alkaline lipase of Penicillium cyclopium PG37 was cloned with four steps of PCR amplification based on different principles. The cloned gene was 1,480 nucleotides in length, consisted of 94 bp of promoter region, and had 6 exons and 5 short introns ranging from 50 to 70 nucleotides. The open reading frame encoded a protein of 285 amino acid residues consisting of a 27‐AA signal peptide and a 258‐AA mature peptide, with a conserved motif of Gly‐X‐Ser‐X‐Gly shared by all types of alkaline lipases. However, this protein had a low homology with lipases of P. camembertii (22.9%), Humicola lanuginosa (25.6%), and Rhizomucor miehei (22.3%) at the amino acid level. The mature peptide‐encoding cDNA was cloned and expressed in Escherichia coli on pET‐30a for confirmation. A distinct band with a M.W. of 33 kDa was detected on SDS‐PAGE. Results of a Western blot analysis and an enzyme activity assay verified the recombinant 33‐kDa protein as an alkaline lipase. Its catalytic properties were not changed when compared with its natural counterpart.