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Similar effects of c 9, t 11‐CLA and t 10, c 12‐CLA on immune cell functions in mice
Author(s) -
Kelley D. S.,
Warren J. M.,
Simon V. A.,
Bartolini G.,
Mackey B. E.,
Erickson K. L.
Publication year - 2002
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-002-0954-7
Subject(s) - splenocyte , immune system , t cell , endocrinology , medicine , biology , tumor necrosis factor alpha , stimulation , secretion , clinical chemistry , immunology
Published results regarding the effects of CLA on immune cell functions have ranged from stimulation to inhibition. In those studies, a mixture of CLA isomers were used, and food intake was not controlled. We have examined whether the discrepancies in the results of earlier studies may be due to the lack of controlled feeding and whether the two isomers of CLA may differ in their effects on immune cell functions. Three groups of C57BL/6 female mice were fed either a control, c 9, t 11‐CLA‐, or t 10, c 12‐CLA (0.5 wt%)‐supplemented diet, 5 g/d, for 56 d. At the end of the study, the number of immune cells in spleens, bone marrows, or in circulation; proliferation of splenocytes in response to T and B cell mitogens; and prosta‐glandin secretion in vitro did not differ among the three groups. Both CLA isomers significantly‐increased in vitro tumor necrosis factor α and interleukin (IL)‐6 secretion and decreased IL‐4 secretion by splenocytes compared to those in the control group. Thus, the two CLA isomers had similar effects on all response variables tested. The discrepancies among the results from previous studies did not seem to be caused by the differences in the isomer composition of CLA used.