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Platelet phospholipids are differentially protected against oxidative degradation by plasmalogens
Author(s) -
Leray Claude,
Cazenave JeanPierre,
Gachet Christian
Publication year - 2002
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-002-0892-4
Subject(s) - phosphatidylethanolamine , lysophosphatidylethanolamine , chemistry , phospholipid , degree of unsaturation , sphingomyelin , glycerophospholipids , membrane , antioxidant , biochemistry , chromatography , phosphatidylcholine
The oxidative degradation of phospholipids in the presence and absence of plasmalogens (plasmenyl phosphatidylethanolamine: PPE) was followed by chemical analysis. Human platelet phospholipids, either intact or after removal of PPE by acid treatment, were oxidized with 28 mM 2,2′‐azobis(2‐amidinopropane di‐HCl in Triton X‐100 micelles (detergent/phospholipid 5∶1, mol/mol). PPE (12% of all phospholipids, mol/mol) disappeared about three times more rapidly than glycerophospholipids, whereas sphingomyelin remained unaltered and the lysophosphatidylethanolamine (lysoPE) generated became progressively more unsaturated. After 60 min oxidation, the FA compositions of PS, PC, and PI were similar in extracts with or without plasmalogens. In contrast, diacyl phosphatidylethanolamine (DPE) became more saturated in the absence of PPE. The rate of phospholipid destruction was always unique to each class, but for all phospholipids slowed down in the presence of PPE. This protective effect increased in the order DPE