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Positional distribution of highly unsaturated fatty acids in triacyl‐ sn ‐glycerols of Artemia nauplii enriched with docosahexaenoic acid ethyl ester
Author(s) -
Ando Yasuhiro,
Oomi Yoshiaki
Publication year - 2001
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-001-0779-4
Subject(s) - docosahexaenoic acid , glycerophospholipids , food science , fatty acid , biology , fish larvae , live food , chemistry , biochemistry , fish <actinopterygii> , botany , larva , polyunsaturated fatty acid , aquaculture , fishery , phospholipid , membrane
This paper presents the positional distribution of fatty acids in triacyl‐ sn ‐glycerols (TAG) of Artemia nauplii used in aquaculture as a live food for marine fish larvae. The nauplii were enriched with docosahexaenoic acid (DHA) ethyl ester (EE) in the form of gelatin‐acacia microcapsules for 4, 18, and 24 h. TAG of the initial, enriched, and unenriched Artemia nauplii were subjected to stereospecific analysis. A remarkable increase of DHA content in the enriched Artemia TAG confirmed the view that DHA‐EE is effectively assimilated and incorporated into the TAG fraction of Artemia nauplii. TAG of the nauplii enriched with 25 mg/L of DHA‐EE contained DHA at concentrations of 5.9–6.8, 4.3–6.0, and 14.3–22.3 mol% in the sn ‐1, sn ‐2, and sn ‐3 positions, respectively. When the nauplii were enriched with 100 mg/L of DHA‐EE, proportions of DHA in the sn ‐1, sn ‐2, and sn ‐3 positions were 5.2–8.6, 3.9–6.0, and 12.2–25.4 mol%, respectively. In all of the enriched Artemia , DHA was preferentially located in the sn ‐3 position followed in sequence by the sn ‐1 and sn ‐2 positions. The lower content of DHA in the sn ‐1 and sn ‐2 positions was consistent with low content of this acid in 1,2‐diacyl‐ sn ‐glycerophospholipids. When fish larvae are reared on Artemia nauplii enriched with LL‐type DHA oil, the larvae feed on DHA esterified in TAG with a positional distribution pattern similar to that of marine mammals ( sn ‐3≫ sn ‐1> sn ‐2) rather than that of fish or marine invertebrates ( sn ‐2≫ sn ‐3> sn ‐1).