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Protein‐precipitating capacity of crude condensed tannins of canola and rapeseed hulls
Author(s) -
Naczk M.,
Amarowicz R.,
Zadernowski R.,
Shahidi F.
Publication year - 2001
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11745-001-0409-6
Subject(s) - canola , rapeseed , titration , chemistry , titration curve , chromatography , protein precipitation , absorbance , bovine serum albumin , precipitation , brassica , zoology , food science , botany , biology , organic chemistry , high performance liquid chromatography , physics , meteorology
The protein‐precipitating potentials (PPP) of soluble condensed tannins (SCT) were determined in hulls from several samples of canola and rapeseed varieties. The PPP were expressed as slopes of lines (titration curves) reflecting the amount of SCT‐protein precipitated vs. the amount of SCT added to the reaction mixture. The slopes ( S p ) of titration curves obtained using the protein‐precipitation assay ranged from 2.96 to 10.91 (absorbance units at 510 nm per mg SCT), and those of titration curves, obtained using the dye‐labeled bovine serum albumin (BSA) assay ( S d ), ranged from 28.1 to 267 (% precipitated BSA per mg SCT). For both assays, a statistically significant ( P ≤0.001) semilogarithmic linear correlation existed between the slopes and the SCT contents in the canola and rapeseed hulls. Higher amounts of SCT‐protein complexes were precipitated at 40°C than at room temperature. Determination of titration curves under standardized conditions (type and concentration of protein, pH and temperature) afforded meaningful differences in the slopes among the range of SCT extracts from canola and rapeseed hulls used in this study.