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Unusual lipid composition of a Bacillus sp. Isolated from Lake Pomorie in Bulgaria
Author(s) -
Carballeira Nstor M.,
Guzmn Aikomari,
Nechev Jordan T.,
Lahtchev Kantcho,
Ivanova Albena,
Stefanov Kamen
Publication year - 2000
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-000-0654-3
Subject(s) - double bond , dimethyl disulfide , derivatization , chemistry , stereochemistry , fatty acid , organic chemistry , high performance liquid chromatography , sulfur
The lipid composition of a Bacillus sp., isolated from Lake Pomorie in Bulgaria, was unusual and consisted of 26 different fatty acids between C 12 and C 26 , with anteiso C 15 −C 17 saturated fatty acids predominating. The furan fatty acid, 10,13‐epoxy‐11‐methyloctadeca‐10,12‐dienoic acid, was also identified, a new finding for this genus. The hydrocarbons consisted of 30 different monounsaturated hydrocarbons, between C 25 and C 30 , with the iso‐iso, iso‐anteiso, anteiso‐anteiso, iso‐normal, and anteiso‐normal methyl branching for odd‐numbered chains, and the iso‐iso, iso‐anteiso, iso‐normal, and anteiso‐normal methyl branching for even‐numbered chains. The double bond positions in these hydrocarbons were determined by dimethyl disulfide derivatization followed by GC‐MS, and the double‐bond cis configuration was confirmed by infrared spectroscopy. Some previously unknown hydrocarbons in bacteria, such as (Z)‐3,21‐dimethyl‐9‐tricosene, (Z)‐3,21‐dimethyl‐10‐tricosene, (Z)‐2,24‐dimethyl‐11‐pentacosene, and (Z)‐2,25‐dimethyl‐13‐hexacosene were identified. Sterols were detected and were based on the sitosterol nucleus.