Premium
Phospholipid identification and quantification of membrane vesicle subfractions by 31 P− 1 H two‐dimensional nuclear magnetic resonance
Author(s) -
Larijani B.,
Poccia D. L.,
Dickinson L. Charles
Publication year - 2000
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-000-0645-4
Subject(s) - phospholipid , vesicle , chemistry , membrane , chromatography , nuclear magnetic resonance spectroscopy , analytical chemistry (journal) , quantitative analysis (chemistry) , nuclear magnetic resonance , biochemistry , stereochemistry , physics
An approach to the direct quantification of phospholipids from two‐dimensional 31 P− 1 H nuclear magnetic resonance (NMR) spectroscopy with isotropic proton mixing has been developed as a general method for phospholipid analysis of minor membrane vesicle subfractions. Membrane vesicles were subfractionated by sedimentation to density equilibrium in a sucrose gradient, and a modified Folch method was employed to extract their phospholipids. The coefficient for the NMR detection efficiency of each phospholipid and the relative mole percentage of the phospholipids present in the membrane vesicles were calculated. We demonstrate low detection limits such that relative concentrations of phospholipids in membrane subfractions may be determined even in the submicromolar range.