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Effect of n−3 fatty acid deficiency on fatty acid composition and metabolism of aminophospholipids in rat brain synaptosomes
Author(s) -
Ikemoto Atsushi,
Ohishi Masayo,
Hata Noriaki,
Misawa Yoshihisa,
Fujii Yoichi,
Okuyama Harumi
Publication year - 2000
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-000-0626-7
Subject(s) - ethanolamine , glycerophospholipid , lipidology , clinical chemistry , docosahexaenoic acid , polyunsaturated fatty acid , fatty acid , biochemistry , chemistry , serine , synaptosome , biology , food science , phospholipid , enzyme , membrane
Docosahexaenoic acid (DHA, 22∶6n−3) is one of the major polyunsaturated fatty acids esterified predominantly in aminophospholipids such as ethanolamine glycerophospholipid (EtnGpl) and serine glycerophospholipid (SerGpl) in the brain. Synaptosomes prepared from rats fed an n−3 fatty acid‐deficient safflower oil (Saf) diet had significantly decreased 22∶6n−3 content with a compensatory increased 22∶5n−6 content when compared with rats fed an n−3 fatty acid‐sufficient perilla oil (Per) diet. When the Saf group was shifted to a diet supplemented with safflower oil plus 22∶6n−3 (Saf+DHA) after weaning, 22∶6n−3 content was found to be restored to the level of the Per group. The uptake of [ 3 H]ethanolamine and its conversion to [ 3 H]EtnGpl did not differ significantly among the three dietary groups, whereas the formation of [ 3 H]lysoEtnGpl from [ 3 H]ethanolamine was significantly lower in the Saf group than in the other groups. The uptake of [ 3 H]serine, its incorporation into [ 3 H]SerGpl, and the conversion into [ 3 H]EtnGpl by decarboxylation of [ 3 H]SerGpl did not differ among the three dietary groups. The observed decrease in lysoEtnGpl formation associated with a reduction of 22∶6n−3 content in rat brain synaptosomes by n−3 fatty acid deprivation may provide a clue to reveal biochemical bases for the dietary fatty acids‐behavior link.