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Production of eicosapentaenoic acid by a recombinant marine cyanobacterium, Synechococcus sp.
Author(s) -
Yu Reiko,
Yamada Akiko,
Watanabe Kazuo,
Yazawa Kazunaga,
Takeyama Haruko,
Matsunaga Tadashi,
Kurane Ryuichiro
Publication year - 2000
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-000-0619-6
Subject(s) - eicosapentaenoic acid , cyanobacteria , synechococcus , docosahexaenoic acid , bacteria , light intensity , fatty acid , biology , chemistry , polyunsaturated fatty acid , food science , biochemistry , botany , genetics , physics , optics
The eicosapentaenoic acid (EPA) synthesis gene cluster from an EPA‐producing bacterium, Shewanella sp. SCRC‐2738, was cloned into a broad‐host range vector, pJRD215, and then introduced into a marine cyanobacterium, Synechococcus sp. NKBG15041c, by conjugation. The transconjugant cyanobacteria produced 3.7±0.2% (2.24±0.13 mg/L) EPA (n‐3) and 2.5 ±0.2% (1.49±0.06 mg/L) eicosatetraenoic acid (n‐3) of the total fatty acids when the cells were cultured at 23°C at a light intensity of 1,000–1,500 Lux. The EPA and eico‐satetraenoic acid contents of the cells were increased to 4.6±0.6% (3.86±1.11 mg/L) and 4.7±0.3% (3.86±0.82 mg/L), and 7.5±0.3% (1.76±0.10 mg/L) and 5.1±0.2% (1.19±0.06 mg/L) when they were cultured at low temperature (18°C) and at lower light intensity (40 Lux), respectively.