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Fatty acid transport across lipid bilayer planar membranes
Author(s) -
RomanoFontes L. G.,
Curi R.,
Peres C. M.,
NishiyamaNaruke A.,
Brunaldi K.,
Abdulkader F.,
Procopio J.
Publication year - 2000
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-000-0491-4
Subject(s) - membrane , chemistry , lipid bilayer , phosphatidylcholine , bilayer , analytical chemistry (journal) , planar , lipid bilayer phase behavior , membrane curvature , solvent , chromatography , phospholipid , organic chemistry , biochemistry , computer graphics (images) , computer science
The transport of palmitic acid (PA) across planar lipid bilayer membranes was measured using a high specific activity [ 14 C]palmitate as tracer for PA. An all‐glass trans chamber was employed in order to minimize adsorbance of PA onto the surface. Electrically neutral (diphytanoyl phosphatidylcholine) and charged (Azolectin) planar bilayers were maintained at open electric circuit. We found a permeability to PA of (8.8±1.9)×10 −6 cm s −1 ( n =15) in neutral and of (10.3±2.2)×10 −6 cm s −1 ( n =5) in charged bilayers. These values fall within the order of magnitude of those calculated from desorption constants of PA in different vesicular systems. Differences between data obtained from planar and vesicular systems are discussed in terms of the role of solvent, radius of curvature, and pH changes.