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Solubilization of phosphatidylcholine unilamellar liposomes caused by alkyl glucosides
Author(s) -
de la Maza A.,
Lopez O.,
Baucells J.,
Gonzalez P.,
Parra J. L.
Publication year - 1998
Publication title -
journal of surfactants and detergents
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.349
H-Index - 48
eISSN - 1558-9293
pISSN - 1097-3958
DOI - 10.1007/s11743-998-0039-x
Subject(s) - chemistry , pulmonary surfactant , liposome , phosphatidylcholine , octyl glucoside , micelle , chromatography , alkyl , bilayer , phospholipid , critical micelle concentration , aqueous solution , lipid bilayer , partition coefficient , solubilization , membrane , organic chemistry , biochemistry
Solubilizing alterations caused by a series of alkyl glucosides (alkyl chain lengths ranging from C 8 to C 12 ) in phosphatidylcholine (PC) unilamellar liposomes were investigated. Surfactant‐to‐phospholipid molar ratios (Re) and bilayer/aqueous phase partition coefficients ( K ) were determined by monitoring changes in static light scattering (SLS) of the system during solubilization. At the two interaction levels investigated (surfactant concentrations producing SLS values of 100 and 0% for each surfactant/PC system studied) the free surfactant concentration for each surfactant was always comparable to its critical micelle concentration (CMC). This indicates that liposome solubilization was mainly ruled by mixed micelle formation. A rise in CMC (or decrease in the surfactant alkyl chain length) resulted in an increase in the ability of these surfactants to saturate or solubilize PC liposomes and, inversely, in an abrupt decrease in their affinity with these bilayer structures. The overall balance of these opposite tendencies shows that the octyl glucoside had the highest ability to saturate and solubilize liposomes (lowest Re values), whereas the dodecyl glucoside exhibited the highest degree of partitioning into liposomes or affinity with bilayer structures (highest K values). From a practical viewpoint, the use of nonyl glucoside reduced approximately 2.5 times the concentration needed to saturate and solubilize 1.0 mM PC liposomes with respect to that needed using the conventional octyl glucoside.