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Preparation and Physicochemical Properties of Lauroyl‐Glutamyl‐Lysil‐Lauroyl‐Glutamate Vesicles
Author(s) -
Fujii Mika,
Fujisaki Tomoko,
Fukuda Toshio,
Wada Yuko,
Shimokawa Kenichi,
Ishii Fumiyoshi
Publication year - 2017
Publication title -
journal of surfactants and detergents
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.349
H-Index - 48
eISSN - 1558-9293
pISSN - 1097-3958
DOI - 10.1007/s11743-017-1951-y
Subject(s) - chemistry , vesicle , citric acid , zeta potential , micelle , pulmonary surfactant , amphiphile , chromatography , chemical engineering , membrane , organic chemistry , biochemistry , aqueous solution , copolymer , nanoparticle , engineering , polymer
We investigate the compositions of vesicle formulation by assembling phase diagrams of the ternary components of lauroyl‐glutamyl‐lysil‐lauroyl‐glutamate (C 12 ‐GLG‐C 12 ) peptide‐based gemini‐type amphiphile/citric acid/water using visual and microscopic observations. To clarify interactions between C 12 ‐GLG‐C 12 and citric acid, turbidity and zeta potential of the mixtures were investigated. To improve the stability of metastable vesicles, appropriate additives were examined by measuring particle size and zeta potential, visual observation and transmission electron microscopy. The turbidity increased and the absolute value of zeta potential decreased with increasing citric acid concentration in C 12 ‐GLG‐C 12 /citric acid solutions. Then, a metastable γ region having vesicles was formed. The stability of vesicles increased with increasing cholesterol concentrations. This result suggests that a coacervation has occurred due to the effective cross‐sectional area decreasing with increasing C 12 ‐GLG‐C 12 hydrophobicity, which was derived from inhibition of the carboxyl group dissociation. Furthermore, we hypothesized that the orientation of cholesterol between vesicle membranes is a contributing factor to improve the vesicle stability. From transmission electron microscopic observations, it was made clear that the vesicles consisting of cholesterol and C 12 ‐GLG‐C 12 at a molar ratio of 2:1 showed multilayered structures. The vesicles consisting of C 12 ‐GLG‐C 12 , cholesterol and citric acid are expected to be used as transdermal drug delivery system carriers, as they can be easily prepared using biocompatible compounds without the use of organic solvents such as chloroform.