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Effect of Surfactants on Catalytic Activity of Diastase α‐Amylase
Author(s) -
Antony Navya,
Balachandran SreedharanNair,
Mohanan Puzhavoorparambil V.
Publication year - 2014
Publication title -
journal of surfactants and detergents
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.349
H-Index - 48
eISSN - 1558-9293
pISSN - 1097-3958
DOI - 10.1007/s11743-013-1531-8
Subject(s) - chemistry , pulmonary surfactant , ammonium bromide , sodium dodecyl sulfate , cationic polymerization , amylase , catalysis , hydrolysis , starch , diastase , sorbitan , sodium , ether , nuclear chemistry , organic chemistry , enzyme , biochemistry , fatty acid , fatty acid ester
Abstract The enzyme amylase is one of the hydrolyzing enzymes used in detergent formulation in order to remove soil based on polysaccharides. The effectiveness of the enzyme depends on its compatibility with other ingredients of the formulation. Among the studied additives, comprising anionic surfactants sodium dodecyl hydrogen sulfate (SDS) and dioctyl sodium sulfosuccinate, the cationic surfactant cetyl trimethyl ammonium bromide (CTAB), nonionic surfactants polyoxyethylene sorbitan monooleate and polyoxyethylene octyl phenyl ether, carboxy methyl cellulose and sodium sulfate, only the anionic surfactant SDS and cationic surfactant CTAB showed catalytic enhancement of α‐amylase. The kinetic parameters, K m and k cat , showed an increase in catalytic activity in the micellar pseudophase. The decrease in optimum temperature from 55 to 30 °C and the shift in optimum pH from 5.5 to 7 on the addition of SDS and CTAB for the hydrolysis of starch are very favorable to enhance the washing characteristics.