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Effect of Sonication on the Thermotropic Behavior of DODAB Vesicles Studied by Fluorescence Probe Solubilization
Author(s) -
Feitosa Eloi,
Benatti Carlos Roberto,
Tiera Marcio José
Publication year - 2010
Publication title -
journal of surfactants and detergents
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.349
H-Index - 48
eISSN - 1558-9293
pISSN - 1097-3958
DOI - 10.1007/s11743-009-1169-8
Subject(s) - chemistry , thermotropic crystal , vesicle , sonication , bilayer , fluorescence , diphenylhexatriene , bromide , pyrene , dipalmitoylphosphatidylcholine , cationic polymerization , fluorescence anisotropy , chromatography , membrane , polymer chemistry , inorganic chemistry , organic chemistry , phospholipid , phosphatidylcholine , polymer , liquid crystalline , biochemistry , physics , quantum mechanics
The effect of sonication on fluorescence probe solubilization in cationic vesicles of dioctadecyldimethylammonium bromide (DODAB) was investigated by steady‐state fluorescence of pyrene (Py), trans ‐diphenylpolyenes—diphenylbutadiene (DPB), diphenylhexatriene (DPH), and their corresponding 4,4′‐dialkyl derivatives 4B4A and 4H4A fluorescence probes. The data indicate that sonication affects the bilayer polarity, the melting temperature ( T m ), and the cooperativity of the melting process due to changes in vesicle morphology. The effect of temperature on the fluorescence intensity and yielding Φ f and anisotropy < r > shows that the ionizable probes 4B4A and 4H4A are solubilized close to the vesicle interfaces, whereas the non‐ionizable DPH and DPB are deeper in the bilayers. Py solubilization indicates that sonicated vesicles exhibit less densely packed bilayers.