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Hard‐Surface Cleaning Using Lipases: Enzyme–Surfactant Interactions and Washing Tests
Author(s) -
Jurado Encarnación,
Bravo Vicente,
Luzón Germán,
FernándezSerrano Mercedes,
GarcíaRomán Miguel,
AltmajerVaz Deisi,
Vicaria José María
Publication year - 2007
Publication title -
journal of surfactants and detergents
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.349
H-Index - 48
eISSN - 1558-9293
pISSN - 1097-3958
DOI - 10.1007/s11743-006-1009-z
Subject(s) - chemistry , lipase , triolein , tributyrin , pulmonary surfactant , hydrolysis , chromatography , cutinase , triacylglycerol lipase , nonionic surfactant , organic chemistry , enzyme , biochemistry
A commercial lipase (E.C. 3.1.1.3) from Thermomyces lanuginosus was studied in order to assess its interaction with commercial nonionic (Findet ® 1214N/16, Findet 1214N/23 and Glucopon ® 650) and anionic (linear alkylbenzene sulphonate; LAS) surfactants, as well as the cleaning action exerted by the enzyme on hard surfaces. Nonionic surfactants seem to prevent or delay enzyme penetration at the interface, thereby decreasing lipase activity. Notably, no inhibitory effect of the anionic surfactant LAS on lipase action was found, higher conversions being achieved after 20 min of enzymatic hydrolysis in the presence of this surfactant than in its absence. A device for testing detersive performance, the so‐called bath–substrate–flow, was used in washing experiments with the lipase at different temperatures with or without surfactant. Employing two different oily stains (tributyrin and triolein), it was found that the lipase by itself increases detergency significantly, preventing the subsequent redeposition of the removed dirt. Expressions relating detersive efficiency to lipase concentration and temperature were obtained using “Statistical Design of Experiments” methodology.