Open AccessLongitudinal FRET Imaging of Glucose and Lactate Dynamics and Response to Therapy in Breast Cancer Cells
Author(s) -
Jianchen Yang,
Tessa Davis,
Anum S. Kazerouni,
YuanI Chen,
Meghan J. Bloom,
HsinChih Yeh,
Thomas E. Yankeelov,
John Virostko
Publication year - 2021
Publication title -
molecular imaging and biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.846
H-Index - 68
eISSN - 1860-2002
pISSN - 1536-1632
DOI - 10.1007/s11307-021-01639-4
Subject(s) - cancer cell , förster resonance energy transfer , chemistry , glucose transporter , glucose uptake , biophysics , biochemistry , medicine , endocrinology , biology , cancer , insulin , fluorescence , physics , quantum mechanics
The reprogramming of cellular metabolism is a hallmark of cancer. The ability to noninvasively assay glucose and lactate concentrations in cancer cells would improve our understanding of the dynamic changes in metabolic activity accompanying tumor initiation, progression, and response to therapy. Unfortunately, common approaches for measuring these nutrient levels are invasive or interrupt cell growth. This study transfected FRET reporters quantifying glucose and lactate concentration into breast cancer cell lines to study nutrient dynamics and response to therapy.