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Nondestructive determination of tannin content in intact individual acorns by near‐infrared spectroscopy
Author(s) -
Takahashi Akiko,
Shimada Takuya,
Kawano Sumio
Publication year - 2011
Publication title -
ecological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.628
H-Index - 68
eISSN - 1440-1703
pISSN - 0912-3814
DOI - 10.1007/s11284-011-0823-6
Subject(s) - tannin , tannic acid , partial least squares regression , calibration , spectroscopy , analytical chemistry (journal) , infrared spectroscopy , near infrared reflectance spectroscopy , near infrared spectroscopy , content (measure theory) , chemistry , infrared , standard error , biological system , materials science , mathematics , chromatography , optics , biology , food science , statistics , physics , organic chemistry , mathematical analysis , quantum mechanics
To examine the effects of tannin content in acorns of Quercus serrata on the fate of individual seeds, we developed a system for nondestructive chemical analysis of single acorns by near‐infrared spectroscopy. We modeled the relationship between the normalized second‐derivative spectra and tannin content by partial least squares regression. We obtained the best calibration model ( r 2 = 0.836; standard error of calibration = 1.90; standard error of validation = 1.43; bias = 0.10). The regression coefficient plots showed that the key band for the model was 938 nm, close to the absorption band of tannic acid in aqueous solution.