Open AccessNef-induced differential gene expression in primary CD4+ T cells following infection with HIV-1 isolates
Author(s) -
Robert L. Furler,
Ayub Ali,
Otto O. Yang,
Douglas F. Nixon
Publication year - 2019
Publication title -
virus genes
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.642
H-Index - 56
eISSN - 1572-994X
pISSN - 0920-8569
DOI - 10.1007/s11262-019-01670-2
Subject(s) - biology , gene , ribosome biogenesis , transcriptome , interferon , viral replication , gene expression , virology , rna , microbiology and biotechnology , interferon stimulated gene , virus , genetics , ribosome , immune system , innate immune system
Almost 80% of viral transcripts during early HIV-1 infection encode the Nef protein, which has been implicated in altering expression of a number of genes. In this study, we infected primary human CD4 + T cells with pseudotyped Nef-containing or Nef-deleted (Δ-nef) NL4-3 virus and used RNA-Sequencing (RNA-Seq) for transcriptomic analysis. Our results showed that the interferon response, IL-15 and JAK/STAT signaling, as well as genes involved in metabolism, apoptosis, cell cycle regulation, and ribosome biogenesis were all altered in the presence of Nef. These early Nef-mediated transcriptional alterations may play a role in priming the host cell for cellular activation and viral replication.