Molecular identification of chlamydial cause of abortion in small ruminants in Jordan | Zendy

Huthaifa Salah Ababneh | Zendy; Mustafa Mohammed Kheir Ababneh | Zendy; Wael M. Hananeh | Zendy; Fawzi Mohammad Alsheyab | Zendy; Khaleel Ibraheem Jawasreh | Zendy; Moath Ahmad Al-Gharaibeh | Zendy; Mohammed M. Ababneh | Zendy

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Molecular identification of chlamydial cause of abortion in small ruminants in Jordan

Author(s) -

Huthaifa Salah Ababneh,

Mustafa Mohammed Kheir Ababneh,

Wael M. Hananeh,

Fawzi Mohammad Alsheyab,

Khaleel Ibraheem Jawasreh,

Moath Ahmad Al-Gharaibeh,

Mohammed M. Ababneh

Publication year - 2014

Publication title -

tropical animal health and production

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.5

H-Index - 49

eISSN - 1573-7438

pISSN - 0049-4747

DOI - 10.1007/s11250-014-0654-x

Subject(s) - abortion , biology , restriction fragment length polymorphism , virology , pregnancy , gene , polymerase chain reaction , genetics

Chlamydophila abortus (Ch. abortus) is the etiological agent of ovine enzootic abortion (OEA) and one of the most common infectious agents of abortion in small ruminants worldwide. RFLP-PCR analysis of the outer membrane protein gene (OMP2 gene) was used for diagnosis and characterization of chlamydial causes of abortion in small ruminants in Jordan. Sixty-six placental tissues and 15 vaginal swabs were collected from aborted ewes and does to identify cause of abortion in Jordan. Thirty-eight placental samples (58 %) and 13 vaginal swabs (87 %) were positive for chlamydial DNA. Shedding of bacteria in vaginal swabs was detected within 7 days after abortion. The results of this study showed that chlamydiosis is one of the important causes of abortion in small ruminants in Jordan. In addition, vaginal swab is an excellent sample for molecular diagnosis of chlamydiosis. DNA sequencing and RFLP analysis of the OMP2 reveal that all chlamydial cause of abortion in small ruminants in Jordan are due to Ch. abortus. While, Ch. pecorum was not detected in any sample. OMP2 gene of the isolated Jordanian strain was identical (100 %) to Ch. abortus FAS strain. In conclusion, Ch. abortus is an important cause of abortion in Jordan; vaginal swab within 7 days of abortion can be used for molecular diagnosis of chlamydiosis in small ruminants.

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