Open AccessHigh frequency electrical stimulation promotes expression of extracellular matrix proteins from human astrocytes
Author(s) -
Jin Sung Jang,
Chan Il Choi,
Jiwon Yi,
Kim A. Butters,
Inyong Kim,
Aditya Bhagwate,
Jin Jen,
Chang Su
Publication year - 2019
Publication title -
molecular biology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 71
eISSN - 1573-4978
pISSN - 0301-4851
DOI - 10.1007/s11033-019-04890-9
Subject(s) - neuroscience , stimulation , deep brain stimulation , astrocyte , extracellular matrix , neuromodulation , neurotransmission , biology , extracellular , matricellular protein , neuroplasticity , synaptic plasticity , microbiology and biotechnology , medicine , central nervous system , biochemistry , receptor , disease , parkinson's disease
Therapeutic benefits of deep brain stimulation (DBS), a neurosurgical treatment for certain movement disorders and other neurologic conditions, are well documented, but DBS mechanisms remain largely unexplained. DBS is thought to modulate pathological neural activity. However, although astrocytes, the most numerous cell type in the brain, play a significant role in neurotransmission, chemical homeostasis and synaptic plasticity, their role in DBS has not been fully examined. To investigate astrocytic function in DBS, we applied DBS-like high frequency electrical stimulation for 24 h to human astrocytes in vitro and analyzed single cell transcriptome mRNA profile. We found that DBS-like high frequency stimulation negatively impacts astrocyte metabolism and promotes the release of extracellular matrix (matricellular) proteins, including IGFBP3, GREM1, IGFBP5, THBS1, and PAPPA. Our results suggest that astrocytes are involved in the long-term modulation of extra cellular matrix environments and that they may influence persistent cell-to-cell interaction and help maintain neuromodulation over time.