Open AccessCyclophosphamide treatment evoked side effects on skeletal muscle monitored by DSC
Author(s) -
D. Lőrinczy
Publication year - 2020
Publication title -
journal of thermal analysis and calorimetry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.521
H-Index - 92
eISSN - 1588-2926
pISSN - 1388-6150
DOI - 10.1007/s10973-020-09388-2
Subject(s) - myosin , cyclophosphamide , skeletal muscle , differential scanning calorimetry , side effect (computer science) , polyneuropathy , chemistry , pharmacology , actin , gastrocnemius muscle , in vivo , drug , chemotherapy , biophysics , medicine , biochemistry , biology , microbiology and biotechnology , computer science , physics , thermodynamics , programming language
Polyneuropathy is defined as a simultaneous malfunction of several peripheral nerves, which could be a side effect of cancer therapy as well. Many kinds of drugs, supposedly cyclophosphamide, also can induce a disease classified as toxic polyneuropathy. It is well known that a severe problem in the locomotor activity can join to it. Recently, we have no enough information about the attacked points in the structure of muscle proteins, as well as about the change in the interaction of myosin actin. In the present study, we analyse this side effect on skeletal muscle ( m. gastrocnemius ) by differential scanning calorimetry (DSC), as an established thermal analysis method, to follow the possible consequence of drug treatment in the most important muscle protein. We used cyclophosphamide-treated in vitro animal model (guinea pig) with a comparable dosage and time handling of human protocol to show evidences of this drug-induced effects. According to our results, we could show a dose-dependent difference between thermal parameters (denaturation temperature and calorimetric enthalpy) of untreated and treated samples assigned to their contractile proteins (actin and myosin), which can be detected by DSC. It proved that we can create new possibilities in the detection and prognosis of expected and unwanted side effects of cyclophosphamide, such as change of locomotor activity joined to polyneuropathy.