Open AccessCombined multi‐band decoupling in biomolecular NMR spectroscopy
Author(s) -
Clemens Anklin,
R. Andrew Byrd
Publication year - 2021
Publication title -
journal of biomolecular nmr
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 106
eISSN - 1573-5001
pISSN - 0925-2738
DOI - 10.1007/s10858-021-00360-2
Subject(s) - decoupling (probability) , chemistry , conceptualization , sensitivity (control systems) , nuclear magnetic resonance spectroscopy , spectroscopy , interference (communication) , biological system , nuclear magnetic resonance , nanotechnology , computer science , physics , electronic engineering , materials science , quantum mechanics , artificial intelligence , telecommunications , engineering , channel (broadcasting) , organic chemistry , control engineering , biology
Multi-resonance NMR experiments are powerful analytical and structural tools. Their conceptualization assumes that RF fields may be combined independently to manipulate spin interactions. However, practical implementation can compromise performance. One limitation is the generation of combination bands when two or more RF fields are applied simultaneously within the NMR probe. The combination bands can lead to significant interference with the detection circuitry. A facile approach to combined multi-band decoupling can resolve these problems and increase sensitivity two-fold (or more), by time sharing the application of the individual frequencies rather than time sharing decoupling and data acquisition.