Open AccessMouse oocyte vitrification with and without dimethyl sulfoxide: influence on cryo-survival, development, and maternal imprinted gene expression
Author(s) -
Clementina Cantatore,
Jenny S. George,
Raffaella Depalo,
Giuseppe D’Amato,
Molly B. Moravek,
Gary D. Smith
Publication year - 2021
Publication title -
journal of assisted reproduction and genetics
Language(s) - English
Resource type - Journals
eISSN - 1573-7330
pISSN - 1058-0468
DOI - 10.1007/s10815-021-02221-1
Subject(s) - vitrification , oocyte , genomic imprinting , andrology , reproductive medicine , biology , dimethyl sulfoxide , gene expression , gene , embryo , genetics , pregnancy , chemistry , medicine , organic chemistry , dna methylation
Oocytes and embryos can be vitrified with and without dimethyl sulfoxide (DMSO). Objectives were to compare no vitrification (No-Vitr), vitrification with DMSO (Vitr + DMSO), and vitrification without DMSO (Vitr - DMSO) on fresh/warmed oocyte survival, induced parthenogenetic activation, parthenogenetic embryo development, and embryonic maternal imprinted gene expression.