Open AccessAgeing and ovarian stimulation modulate the relative levels of transcript abundance of oocyte DNA repair genes during the germinal vesicle-metaphase II transition in mice
Author(s) -
Fabrizzio Horta,
Aravind Ravichandran,
Sally Catt,
Beverley Vollenhoven,
Peter Temple-Smith
Publication year - 2020
Publication title -
journal of assisted reproduction and genetics
Language(s) - English
Resource type - Journals
eISSN - 1573-7330
pISSN - 1058-0468
DOI - 10.1007/s10815-020-01981-6
Subject(s) - germinal vesicle , oocyte , biology , andrology , endocrinology , medicine , ageing , in vitro maturation , dna repair , gene , embryo , genetics
Oocyte quality and reproductive outcome are negatively affected by advanced maternal age, ovarian stimulation and method of oocyte maturation during assisted reproduction; however, the mechanisms responsible for these associations are not fully understood. The aim of this study was to compare the effects of ageing, ovarian stimulation and in-vitro maturation on the relative levels of transcript abundance of genes associated with DNA repair during the transition of germinal vesicle (GV) to metaphase II (MII) stages of oocyte development.