Open AccessBioinformatic identification of euploid and aneuploid embryo secretome signatures in IVF culture media based on MALDI-ToF mass spectrometry
Author(s) -
Ricardo Jorge Pais,
Fady I. Sharara,
Raminta Zmuidinaite,
Stephen A. Butler,
Sholeh Keshavarz,
Ray K. Iles
Publication year - 2020
Publication title -
journal of assisted reproduction and genetics
Language(s) - English
Resource type - Journals
eISSN - 1573-7330
pISSN - 1058-0468
DOI - 10.1007/s10815-020-01890-8
Subject(s) - embryo , biology , ploidy , embryo culture , mass spectrometry , genotype , embryo quality , selection (genetic algorithm) , computational biology , andrology , genetics , in vitro fertilisation , cryopreservation , chemistry , medicine , computer science , gene , chromatography , artificial intelligence
Embryo genotyping in IVF clinics aims to identify aneuploid embryos, and current methodologies rely on costly, invasive and time-consuming approaches such as PGT-A screening. MALDI-ToF-based mass spectral analysis of embryo culture has been demonstrated to be a non-invasive, affordable and accurate technique that is able to capture secretome profiles from embryo culture media extremely quick. Thus, aneuploid embryo genotypes can be distinguished from euploids from these profiles towards the development of novel embryo selection tools.