Open AccessLoss of methylation of H19-imprinted gene derived from assisted reproductive technologies can be mitigated by cleavage-stage embryo transfer in mice
Author(s) -
Shuqiang Chen,
Meizi Zhang,
Li Li,
Ming Wang,
Yuhua Shi,
Hengde Zhang,
Bin Kang,
Na Tang,
Bo Li
Publication year - 2019
Publication title -
journal of assisted reproduction and genetics
Language(s) - English
Resource type - Journals
eISSN - 1573-7330
pISSN - 1058-0468
DOI - 10.1007/s10815-019-01575-x
Subject(s) - genomic imprinting , methylation , blastocyst , biology , andrology , embryo , imprinting (psychology) , intracytoplasmic sperm injection , dna methylation , somatic cell nuclear transfer , embryo transfer , bisulfite sequencing , inner cell mass , in vitro fertilisation , embryogenesis , genetics , gene , medicine , gene expression
Studies on rodents have shown that assisted reproductive technologies (ARTs) are associated with perturbation of genomic imprinting in blastocyst-stage embryos. However, the vulnerable developmental window for ART influence on the genomic imprinting of embryos is still undetermined. The purpose of this study was to establish the specific embryonic development stage at which the loss of methylation of H19 imprinting control regions (ICRs) was caused by ART occurrence. Additionally, we explored protocols to safeguard against possible negative impacts of ART on embryo H19 imprinting.