Open AccessRemoval of DNA-fragmented spermatozoa using flow cytometry and sorting does not improve the outcome of intracytoplasmic sperm injection
Author(s) -
Christian De Geyter,
Ursula Gobrecht-Keller,
Astrid Ahler,
Manuel Fischer
Publication year - 2019
Publication title -
journal of assisted reproduction and genetics
Language(s) - English
Resource type - Journals
eISSN - 1573-7330
pISSN - 1058-0468
DOI - 10.1007/s10815-019-01571-1
Subject(s) - andrology , dna fragmentation , intracytoplasmic sperm injection , semen , pregnancy rate , biology , male infertility , sperm , flow cytometry , live birth , miscarriage , pregnancy , semen analysis , infertility , gynecology , apoptosis , medicine , immunology , genetics , programmed cell death
The DNA fragmentation in sperm is associated with reduced outcome in assisted reproduction. Using YoPro1 as the staining dye and flow cytometry and sorting (FACS), the number of spermatozoa with DNA fragmentation can be lowered to 5%. Can the cumulative outcome of ICSI be improved using FACS?