Open AccessIn vitro survival of follicles in prepubertal ewe ovarian cortex cryopreserved by slow freezing or non-equilibrium vitrification
Author(s) -
Yann Locatelli,
Laure Calais,
Nicolas Duffard,
Lionel Lardic,
Danielle Monniaux,
P. Piver,
Pascal Mermillod,
Michael J. Bertoldo
Publication year - 2019
Publication title -
journal of assisted reproduction and genetics
Language(s) - English
Resource type - Journals
eISSN - 1573-7330
pISSN - 1058-0468
DOI - 10.1007/s10815-019-01532-8
Subject(s) - cryopreservation , ovarian cortex , vitrification , andrology , folliculogenesis , biology , fertility preservation , ovarian follicle , ovarian tissue cryopreservation , embryo culture , in vitro maturation , follicle , reproductive technology , follicular phase , population , endocrinology , embryo , oocyte , microbiology and biotechnology , medicine , fertility , environmental health
Vitrification is a well-accepted fertility preservation procedure for cryopreservation of oocytes and embryos but little is known regarding ovarian tissue, for which slow freezing is the current convention. The aim of the present study was to assess the efficiency of non-equilibrium vitrification compared to conventional slow freezing for ovarian cortex cryopreservation.