Co-localization of LTBP-2 with FGF-2 in fibrotic human keloid and hypertrophic scar | Zendy

Mohamed Arshad Mohamed Sideek | Zendy; Abdulrahman Teia | Zendy; Zlatko Kopecki | Zendy; Allison J. Cowin | Zendy; Mark Gibson | Zendy

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Co-localization of LTBP-2 with FGF-2 in fibrotic human keloid and hypertrophic scar

Author(s) -

Mohamed Arshad Mohamed Sideek,

Abdulrahman Teia,

Zlatko Kopecki,

Allison J. Cowin,

Mark Gibson

Publication year - 2015

Publication title -

journal of molecular histology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.665

H-Index - 58

eISSN - 1567-2387

pISSN - 1567-2379

DOI - 10.1007/s10735-015-9645-0

Subject(s) - hypertrophic scar , keloid , fibrillin , immunostaining , fibroblast , extracellular matrix , elastin , pathology , chemistry , immunohistochemistry , fibroblast growth factor , scars , medicine , biochemistry , receptor , in vitro

We have recently shown that Latent transforming growth factor-beta-1 binding protein-2 (LTBP-2) has a single high-affinity binding site for fibroblast growth factor-2 (FGF-2) and that LTBP-2 blocks FGF-2 induced cell proliferation. Both proteins showed strong co-localisation within keloid skin from a single patient. In the current study, using confocal microscopy, we have investigated the distribution of the two proteins in normal and fibrotic skin samples including normal scar tissue, hypertrophic scars and keloids from multiple patients. Consistently, little staining for either protein was detected in normal adult skin and normal scar samples but extensive co-localisation of the two proteins was observed in multiple examples of hypertrophic scars and keloids. LTBP-2 and FGF-2 were co-localised to fine fibrous elements within the extracellular matrix identified as elastic fibres by immunostaining with anti-fibrillin-1 and anti-elastin antibodies. Furthermore, qPCR analysis of RNA samples from multiple patients confirmed dramatically increased expression of LTBP-2 and FGF-2, similar TGF-beta 1, in hypertrophic scar compared to normal skin and scar tissue. Overall the results suggest that elevated LTBP-2 may bind and sequester FGF-2 on elastic fibres in fibrotic tissues and modulate FGF-2's influence on the repair and healing processes.

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