Open AccessLncRNA-NNT-AS1 contributes to the progression of glioma by miR-582-5p/EZH2 axis
Author(s) -
Tonglin Pan,
Mei Xue
Publication year - 2021
Publication title -
cytotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.488
H-Index - 62
eISSN - 1573-0778
pISSN - 0920-9069
DOI - 10.1007/s10616-021-00471-6
Subject(s) - glioma , cancer research , long non coding rna , biology , microrna , rna , genetics , gene
Increasing studies have shown that long non-coding RNAs (lncRNAs) had crucial regulatory roles in many diseases. Nevertheless, the biological relevance and mechanisms of the NNT-AS1 in gliom remain poorly understood. In the present study, NNT-AS1 expression was up-regulated in the glioma cell lines. Functional assays demonstrated that depletion of NNT-AS1 could notably suppress the proliferation, migration, invasion and EMT of U87MG and A172 cells. In addition, miR-582-5p was predicted to be a target of NNT-AS1 and EZH2 was predicted to be a target of miR-582-5p by bioinformatics software, which was further confirmed by luciferase reporter assay. Additionally, results of recue assays proofed that NNT-AS1/miR-582-5p/EZH2 axis aggravated the malignant behaviors of glioma. Ultimately, our findings revealed that NNT-AS1 contributes to progression via targeting miR-582-5p/EZH2 axis, revealing NNT-AS1 as a latent effective target for the diagnosis and treatment of glioma patients.