Open AccessCharacteristics of apoptosis induction in human breast cancer cells treated with a ceramidase inhibitor
Author(s) -
Hüseyin İzgördü,
Canan Vejselova Sezer,
Emre Çömlekçi,
Hatice Mehtap Kutlu
Publication year - 2020
Publication title -
cytotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.488
H-Index - 62
eISSN - 1573-0778
pISSN - 0920-9069
DOI - 10.1007/s10616-020-00436-1
Subject(s) - ceramide , apoptosis , sphingolipid , annexin , cancer , cancer cell , programmed cell death , breast cancer , cancer research , microbiology and biotechnology , biology , cell cycle , mitochondrion , chemistry , biochemistry , genetics
Cancer is a complex disease with high mortality rates. Breast cancer is one of the most fatal diseases both for men and woman. Despite the positive developments on cancer treatment, a successful treatment agent/method has not been developed, yet. Recently, cancer research has been involved in sphingolipid metabolism. The key molecule here is ceramide. Ceramides mediate growth suppress, apoptosis and aging regulation. Ceramidases metabolize ceramide and decrease its level in cells and cause escape the death. Inhibition of ceramidases as new targets for cancer treatment is shown in the literature. Herein, we found that d-erythro-MAPP and its nanoparticle formulation, reduce the viability of MCF-7 cells in a dose-dependent manner with IC 50 value of 4.4 µM, and 15.6 µM, respectively. Confocal and transmission electron microscopy results revealed apoptotic morphological and ultrastructural changes for both agents. Apoptosis and cell cycle arrest were supported by annexin-V, mitochondrial membrane potential changings and cell cycle analysis, respectively.