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Tetrahydrobiopterin treatment reduces brain L‐Phe but only partially improves serotonin in hyperphenylalaninemic ENU1/2 mice
Author(s) -
Scherer Tanja,
Allegri Gabriella,
Sarkissian Christineh N.,
Ying Ming,
GrischChan Hiu Man,
Rassi Anahita,
Winn Shelley R.,
Harding Cary O.,
Martinez Aurora,
Thöny Beat
Publication year - 2018
Publication title -
journal of inherited metabolic disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 102
eISSN - 1573-2665
pISSN - 0141-8955
DOI - 10.1007/s10545-018-0150-y
Subject(s) - tetrahydrobiopterin , serotonin , endocrinology , medicine , metabolic disease , chemistry , neuroscience , pharmacology , biology , receptor , nitric oxide synthase , nitric oxide
Hyperphenylalaninemia (HPA) caused by hepatic phenylalanine hydroxylase (PAH) deficiency has severe consequences on brain monoamine neurotransmitter metabolism. We have studied monoamine neurotransmitter status and the effect of tetrahydrobiopterin (BH 4 ) treatment in Pah enu1/enu2 (ENU1/2) mice, a model of partial PAH deficiency. These mice exhibit elevated blood L‐phenylalanine (L‐Phe) concentrations similar to that of mild hyperphenylalaninemia (HPA), but brain levels of L‐Phe are still ~5‐fold elevated compared to wild‐type. We found that brain L‐tyrosine, L‐tryptophan, BH 4 cofactor and catecholamine concentrations, and brain tyrosine hydroxylase (TH) activity were normal in these mice but that brain serotonin, 5‐hydroxyindolacetic acid (5HIAA) and 3‐methoxy‐4‐hydroxyphenylglycol (MHPG) content, and brain TH protein, as well as tryptophan hydroxylase type 2 (TPH2) protein levels and activity were reduced in comparison to wild‐type mice. Parenteral L‐Phe loading conditions did not lead to significant changes in brain neurometabolite concentrations. Remarkably, enteral BH 4 treatment, which normalized brain L‐Phe levels in ENU1/2 mice, lead to only partial recovery of brain serotonin and 5HIAA concentrations. Furthermore, indirect evidence indicated that the GTP cyclohydrolase I (GTPCH) feedback regulatory protein (GFRP) complex may be a sensor for brain L‐Phe elevation to ameliorate the toxic effects of HPA. We conclude that BH 4 treatment of HPA toward systemic L‐Phe lowering reverses elevated brain L‐Phe content but the recovery of TPH2 protein and activity as well as serotonin levels is suboptimal, indicating that patients with mild HPA and mood problems (depression or anxiety) treated with the current diet may benefit from supplementation with BH 4 and 5‐OH‐tryptophan.