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Chemical glycomics enrichment: imaging the recycling of sialic acid in living cells
Author(s) -
Gilormini Pierre André,
Lion Cédric,
Vicogne Dorothée,
Guérardel Yann,
Foulquier François,
Biot Christophe
Publication year - 2018
Publication title -
journal of inherited metabolic disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 102
eISSN - 1573-2665
pISSN - 0141-8955
DOI - 10.1007/s10545-017-0118-3
Subject(s) - glycomics , glycoconjugate , sialic acid , biochemistry , glycan , glycosylation , chemistry , oligosaccharide , biology , microbiology and biotechnology , glycoprotein
The development of metabolic oligosaccharide engineering (MOE) over the past two decades enabled the bioimaging studies of glycosylation processes in physio‐pathological contexts. Herein, we successfully applied the chemical reporter strategy to image the fate of sialylated glycoconjugates in healthy and sialin‐deficient patient fibroblasts. This chemical glycomics enrichment is a powerful tool for tracking sialylated glycoconjugates and probing lysosomal recycling capacities. Thus, such strategies appear fundamental for the characterization of lysosomal storage diseases.